Led by Principal Investigator, Dr. Richard Ulevitch at the Scripps Research Institute, Systems Immunity is a consortium of pioneers in the fields of Forward Genetics and Systems Biology.
The Scripps Research Institute
Dr. Ulevitch provides overall guidance and oversight of the program. He also directs the Human Correlation Core, translating the findings from the murine studies onto human biology.
Seattle Children’s Center for Global Infectious Disease Research
Dr. Aderem’s laboratory is using systems biology approaches to understand the molecular regulation of innate immune responses. These studies also predict genes likely to regulate immune responses and are being used to inform the breeding and screening strategies in the Genetics Cores at ANU and UTSW. The lab also conducts various in vitro phenotypic screens on macrophages and dendritic cells from mice harboring ENU mutations to complement the in vivo screens in Genetics Cores.
University of Texas Southwestern Medical Center
Dr. Beutler’s group has developed a forward genetics pipeline that integrates next-generation sequencing with computational tools to drastically reduce the time needed to identify phenotypic ENU-induced mutations. In this project, he will use this approach to identify critical regulators of innate immune responses. He also directs the Mutagenetix repository of mouse mutations and, in concert with Dr. Goodnow’s team at the Autralian Phenomics Facility, will annotate each characterized mutation and make this program resource available to the broader scientific community.
Australian National University
Dr. Goodnow is Chief Scientific Officer of the Australian Phenomics Facility and Head of the Department of Immunology at the John Curtin School of Medical Research. His group is focusing on understanding control systems governing short vs long-term humoral and cellular immunity. This involves a strategy that combines high-throughput sequencing of ENU-induced single nucleotide variants (SNVs) and systems biology to accelerate phenotypic screening by selectively enriching for mutations in SNVs in genes predicted to impact adaptive immune responses.
Dr. Nolan is a pioneer in the development of innovative systems approaches that utilize single-cell measurements to define immune cell lineages and signaling networks. The Nolan Lab is employing a newly developed mass spectrometry/flow cytometric hybrid technology (CyTOF) to quantitatively characterize the impact of ENU-induced mutations on immune signaling networks. Nolan Lab